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RayBiotech's sandwich ELISA kits are pre-coated 96-well strip plates. If you would like to split up your plate testing, each strip includes 8 wells so you can easily split it up in groupings of 8.

We recommend covering the slide during the overnight incubation. Both steps are done together. For more questions about your specific kit, please contact technical support. The information can be found on our website under "contact us." Please provide the kit catalog number during your correspondence to facilitate communication.

Glad it was helpful! :) We appreciate your feedback and will take it into consideration.

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Thanks for the comment! Yes the last PC will be trivial or nearly 0 when n<k, though PCA will still returning the n PCs. It is correct that there will be n-1 reporting no-trivial PC when n<k.

For our ELISA kits, we use 96 well plates and a volume incubation of 100uL. If referring to running 24 wells in 3 strips, the conditions are the same. If you're referring to a full 24 well plate then development and use can be accomplished with 1mL per well volume.

The frequency depends on your experiment. For us, it's protein expression levels.

Hello! The number of cytokines measured depends on the array. Antibody spots are evenly printed on the membrane. The number of measured targets can range from 5 - 507. The experimental run volume for membrane arrays is 1mL per sample. We recommend a dilution factor of at least 2-fold. This leaves you with a maximum volume requirement of 500uL per sample.

Happy you liked it :)

Glad you like it :)

Whether you need a laminar flow hood for your ELISA experiment depends on various factors. ELISA involves handling sensitive samples and requires aseptic conditions to prevent contamination. If you're working with infectious agents, cell cultures, or potentially hazardous substances, a laminar flow hood is essential to maintain a sterile environment and protect both the experiment and the researcher. Additionally, a laminar flow hood aids in preventing cross-contamination between samples and ensures accurate results. However, if your ELISA experiment involves only non-infectious and non-hazardous materials, a well-maintained and clean laboratory bench typically suffices. This video assumes a well maintained and clean laboratory environment is used to run the experiment and does not demonstrate handling infectious material. Ultimately, the decision should consider the experiment's complexity, potential risks, and adherence to best laboratory practices.

This is a sandwich ELISA. We offer a large catalog of other ELISA types on our webpage. We don't have video content for them yet.

We admit our microphone could be better. Thank you for bearing with us as we pilot this project. Did you find the visuals and subtitles helpful? We are open to hearing what more we could do to help demonstrate this technique. Feel free to email info@raybiotech.com for more video requests.

Pipette washing is a common practice and a widely acceptabled way to remove liquid. Many labs will use a clean pipette tip hooked up to a vacuum to suction out liquid. When doing so, please tilt the plate at an angle and be careful not to scrape the bottom of the well with the pipette tip. It's best if an experienced team member demonstrates this technique prior to a new user attempting it for the first time. If this is not possible, then it is best to blot on tissue paper to ensure all liquid is removed. The goal is to remove all liquid while maintaining the integrity of each well during the removal step.

What kind of additional information or demonstration do you think would make it easier?

There are two free densitometry softwares available to use with the image files produced from scanning (L-Series or C-series) Image-J: imagej.nih.gov/ij/index.html Image Studio Lite: www.licor.com/bio/products/software/image_studio_lite/ We provide an excel analysis tool that helps analyze the data once it's extracted. Check out the documents tab on this kit -> www.raybiotech.com/human-cytokine-array-c5-aah-cyt-5

Both ELISA and Western blot use antibodies to detect proteins. ELISAs require an antibody pair where both antibodies detect the target protein to provide a signal. A western separates proteins by size and uses one antibody to detect the protein and another antibody to detect that antibody. The ELISA and IQELISA technique uses a 96-well platform that is typically more user friendly and allows more sample throughput per experiment. Additionally, quantitative data can be obtained on a routine basis with ELISA and IQELISA whereas with the western blot it can be more demanding.

You may touch the ends of the well strips and the sides of the plate with gloved hands. To maintain a sterile working solution avoid touching the individual wells, even if you have gloves on. You can run liquid down the sides of the wells with a sterile pipette tip.

@@RayBiotech Is it direct or indirect or compittive or sandwich elisa? pleasetell me.

@@408_mohitpanchal2 sandwich elisa

In think YOU are the one who needs to review the p-value definition

@@alecambo well said

I think you are very rude and contribute absolutely nothing to this topic, and this video was perfectly done. She did an excellent job by explaining the concepts clearly

@@sunnyyoda this discussion is above your pay grade. if u don't understand stat, its fine but just don't go around commenting on issues whose fundamentals you are not aware of

Read the instructions. Faint line, no matter how faint, means positive . Speedy recovery.

Means you are positive for active or recent COVID infection or had a tremendous response to the vaccine. Most folks are IgG only positive after vaccine or infection given time.